Melanin synthesis inhibition

In B16F10 melanoma cells, PAW treatment significantly reduced melanin content.

Reduced melanin content

1.8× stronger effect
than arbutin

MELANIN INHIBITION

Melanin synthesis inhibition and reduced intracellular melanin

Compared with arbutin

PAW treatment significantly reduced melanin synthesis in B16F10 melanoma cells,
showing inhibition similar to or stronger than arbutin, a standard whitening agent.

Visual observation of melanin in cell pellets

B16F10 (mouse melanoma cell)

Con PAW+ Arbutin

Intracellular melanin content – vs. arbutin

Con	100 %
PAW⁺ 1,000 ppm	34.5 %
Arbutin 1 mM	62.3 %

Melanin content – by concentration

Con	100 %
PAW⁺ 100 ppm	78.1 %
PAW⁺ 500 ppm	67.1 %
PAW⁺ 1,000 ppm	40.4 %

Key findings

Melanin content

Tyr gene

Tyrosinase

PAW significantly reduced the expression of key melanogenesis factors,
showing whitening effects similar to or better than arbutin.